FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408852
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAAAT7552.569041E-49.5661151
CTTTCGA10004.917958E-69.3891421
CTCAAGA10509.131976E-68.942041
CTCCCCG7900.0041074518.2280711
CTCCACT8050.0048656058.0747521
ATTAAAC8600.009041597.53526832
CAACGGA14556.957099E-56.9494051
CTTCGAT15208.875904E-46.17706781
CTTCAAT20352.2370117E-56.03346871
CTTTATT22001.1661225E-55.90925071
CAACAAA18853.138388E-45.74728161
CATCAAG28303.8741655E-65.35939071
CTTTGAT20600.00103661295.2590421
CTTTGAA20800.00117913275.2084741
ATCAAAT21050.00138227755.1466161
CCTGAAA18500.0091141695.0752121
Produced by FastQC (version 0.11.5)