FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493248
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTT1800.00447134920.0065981
CTCATGG3600.00101395914.0046191
GGGGGGG650.00665634613.760606135-139
ATTTGCG4250.003616996811.8254436
CATAGAA4907.6333055E-411.758981
CTTCTAT6455.35406E-511.1664731
AGTTGAT5200.001255509211.0805781
CTCCATT5250.001359975410.9750481
CCTCTTT8055.0065527E-48.9470511
CTTATTT7450.00247258488.7008551
CTTTTAT11703.940624E-68.6182271
CTTCTTT15952.3775036E-46.3218341
CTTTTCT12600.00561021456.2877881
Produced by FastQC (version 0.11.5)