FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1314634
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAT4250.003177841412.0342311
CCCCAAC7200.00159490749.13312151
CTCAGTT6450.0066560649.0623221
CTCCAAC10356.544596E-58.4713021
CCACTAT7800.00329773278.4305731
CTCAATA11853.6132726E-58.0155671
CTCTGAA9150.00155213817.9852431
CTCCGCT9350.00192056197.8144361
TTAGATT8600.0087716717.5608192
CCCGCTT8700.0087961627.55844641
CTCAGGA8800.0097404437.4725541
CTCCCAT12859.896093E-57.3917881
TTTTACG12801.0858229E-47.3365547
CTATAAC10000.00371042527.30649761
CTCTATT12053.8145852E-47.27618031
CTTATAC12154.1940884E-47.21629431
ATCCACT10500.00596402866.9585691
GTCAAAT11650.0021302436.89883951
CTTTCAC18052.9049388E-66.8814661
CTCAAAT10650.00684251736.86056141
Produced by FastQC (version 0.11.5)