FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605058
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAACT9302.072983E-59.3419421
CACGCAA8851.3012828E-48.9554259
CTAAGAT8155.3928694E-48.8834421
CTCTACT8150.0053200377.9950971
CTCACGG12256.128422E-57.6832711
CCCAGCT13953.8710918E-57.26595451
ATCAAAT23502.1937012E-97.08596231
CTTTTAT29952.5465852E-116.7686191
CTCAAAA20354.406993E-76.759711
CTCTTCG18655.591126E-66.59946871
CTTAAAT15651.7465017E-46.4766811
CCTCAAC13850.00206027956.27292871
CTTCAAT24803.2133357E-76.13064961
CTTCGTT16100.00171984915.8459671
CTTCTAC18853.037585E-45.7612771
CTACGAT15100.0054065225.75364641
CTTCAAG28256.036953E-75.6382341
CTCTTTT35007.363269E-95.58514641
CTCCAAA16950.00317134575.5528071
CCTGTTT22509.948063E-55.47022631
Produced by FastQC (version 0.11.5)