FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences499986
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTAAA3350.00996972212.9418546
CGTTCAG5400.003966217411.679644145
CTAAAAA6103.7250627E-410.6986041
CTTAAAT4800.00831607310.5747771
CTTCAAT7903.49033E-510.0967131
CTGAAGA6100.0044679169.509871
CTTTGAA7200.00170515359.06409551
GTTTTGT6650.0090815538.7233391
CTTATTT7750.00332495928.4208361
ATTGGAA7800.00363738448.3375418
CTTTCAT8750.00107265428.2871721
AATTGAA7900.0040804068.2320025
ATAAGAA9250.00185677.83921771
CTTTTTT18657.2630974E-76.99855041
CTTCTTT14900.00456545835.8399541
Produced by FastQC (version 0.11.5)