FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1130866
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGAT11650.0021128746.9039941
GTCTTTG12400.00406752766.4864141
CTTCAAT15408.951213E-46.17243151
CAAAGAT16954.3055083E-46.03937441
CAAATCC14950.00495410345.798783
CTTCCGG18300.00694981965.19428631
CTTTCCG21350.00141525445.13720661