FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1421384
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAA6354.1593525E-511.4499411
CTCCAAG9000.00138414638.0785691
GTTAAAT9250.00181346137.860231
GTCAGTG8350.00712042637.7393438
CTCAGAT8750.0096731797.4784471
CCTATAT10950.00116477677.3039121
TAGATTT11750.00279986936.72206833
TTGAACT15601.8690294E-46.44280772
AGTTGAT12700.00553874076.29746771
CCTCCTT21003.2699252E-55.88581471
CGAATTT14950.0053035345.76352453
CTTTAAT16450.00211282075.74585151
GGCATAT17150.00346460435.51132731
TTTCACG17200.0041561755.42703967
ATTCAAT20559.1803E-45.30708931
TTATTCT23301.902981E-45.2389123
CTTGAAA23952.3812393E-45.16083961
Produced by FastQC (version 0.11.5)