FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1249764
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT5750.021.1076979
CACACGT5353.6379788E-1220.016927
ACACGTC6355.2750693E-1116.8646498
AGAGCAC7351.8189894E-1216.5167873
GAGCACA8552.1827873E-1114.1986434
AGCACAC7951.5297701E-913.4715825
GCACACG9853.6801794E-810.8721346
CACAAAT10551.01043785E-48.1617521
CCTCAAT9100.00175733787.88520861
AAGAGCA15452.9546609E-67.400332
CTTCGAT12701.0702539E-47.34504031
CAAGATC9800.00380910197.28681854
CCCGCAT12356.2175846E-46.9721841
GAAGAGC15803.023604E-56.8122211
CTGCAAT19101.2913752E-66.7622891
CCAAAAT13150.00126901056.5480211
GTATTAT13250.009848485.9570521
CGTCCAC15400.00741550655.591331
Produced by FastQC (version 0.11.5)