FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487188
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGCACACGTCTGAACTCCAGTCACTCCAATCGATCTCGTATGCCGT5750.11802425347093935TruSeq Adapter, Index 6 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC2700.047.3860248
CACACGT2750.046.5244647
CACGTCT3200.042.203189
AGCACAC3150.038.356185
GCACACG3450.037.0847176
GAGCACA3650.033.101914
AGAGCAC3950.032.3871233
GAAGAGC6800.019.9380911
AAGAGCA6600.019.3911652
CAATCGA2755.7716534E-99.32403430-34
CCAATCG3156.730261E-109.04447830-34
CATCTAA1450.00218545378.8228860-64
TCACTCC3403.092282E-108.79371225-29
CAGTCAC2951.9183062E-88.69189620-24
GTCACTC3002.554043E-88.54703120-24
CTCGTAT3208.511051E-98.45835-39
AGTCACT2951.771241E-78.20901320-24
CTCCAGT3453.3600372E-87.829638515-19
CTGAACT4101.5243131E-97.62830810-14
TCGTATG3802.2451786E-87.496624540-44
Produced by FastQC (version 0.11.5)