FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657125
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAG2600.001866774916.6548335
CTCAAGA3900.00167093913.1083611
CTTCAAC7702.7941118E-49.48471451
CCTTCGC6500.0071097578.9885911
CTTCAAA10550.00626220046.9224931
CAAAAAG15950.0013815975.95247551
Produced by FastQC (version 0.11.5)