FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3036872
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAATCC18559.444557E-87.3842093
TAGCCTG11803.501219E-47.3315129
CCTAAAT11100.00140055897.17876771
GTTTAAT21451.5195474E-76.75433971
ACTACAC13000.0077180076.0998928
GTCCGCG13350.0096600695.9688631
CAACAAT37108.403731E-105.6624491
CTTAAAT28003.0267165E-65.43302151
CCCGAAT17850.00580193755.27576351
CTGAAAT42901.0586518E-95.2346131
CCTCGGA18000.00639970465.2317991
ATCTTAC24253.2599317E-45.05370666
Produced by FastQC (version 0.11.5)