FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139625
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCTA3250.008188223513.3355678
ATGGACC5008.8396575E-411.5574916
CCCAGTT7351.7443408E-49.9407871
CTCAATT5900.0031912059.907091
CCCCCCC1604.4771124E-49.0514555125-129
ATTGGAT10054.419382E-47.9971411
CTTTAAT14659.498672E-77.97977161
CTTTAAG10255.45981E-47.84109971
CTCATCT10658.2212425E-47.5465971
CTTTCAT19853.1268428E-87.3616911
CTTCTTA10950.00110510697.3398421
CTCCAAT10150.0042890597.19850061
CTAAAAT10200.0044992557.1632141
CTTCAAA21351.1884549E-76.84447671
CAACGAT11050.0097656656.6121981
CTTCAAG15701.6148946E-46.51533131
CTCTTTT25152.2648019E-65.8103211
CTTTTAT22951.909023E-55.73057171
CTTTTAA16750.0024697365.67071
CCTCATC16000.009254325.4798591
Produced by FastQC (version 0.11.5)