FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948772
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGG6806.077109E-611.8256171
TCCGCTA5008.86258E-411.5535489
CCCAAAT5902.5425066E-411.1514281
CCCTCAT6100.004184229.5873851
CTCCCAT6500.00705774978.9973931
CCCAGAA9903.858765E-58.8610691
TCAATTT12751.2064436E-57.9314143
CCCAAAA9550.00235072317.65484951
CTCTTTT22103.918103E-97.27730231
CTTCAAC11200.00139576377.17983871
TAAATCC11100.00144122487.1581783
CTTTGAT17501.768416E-67.1015131
CTCCTTG11850.0025323816.7860081
CTCAAAA13009.014455E-46.74804451
CTTCCAT17401.2324263E-56.7221891
CAACAAG10900.0085132246.7067721
CTTCAAT14303.652231E-46.6458011
CACAAAG11000.0092968616.6458011
CAATAAA15501.3562576E-46.60292531
CTTCATT15651.5374688E-46.53963761
Produced by FastQC (version 0.11.5)