FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507698
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCATT4502.9758667E-919.3363511
ATCTAAT2952.1205834E-417.2060781
CTCATTG4650.00673673410.8791122
CTTAAAA8154.916408E-59.7868041
CTGAAAG8406.845319E-59.495531
CCTCTTT8851.2097163E-49.0127071
CTCAAAA7300.00193363618.9397521
CTCCTTT9753.4521703E-48.1807641
CTTTTGA8550.0080428657.63277051
Produced by FastQC (version 0.11.5)