FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3834565
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTT19750.09.591891
CTCAATT25050.08.7259251
CTCAAAC30250.08.1893891
CTCAAAT36250.08.0398871
CTCCGAT31350.07.9020421
CCCCAAT21653.43789E-107.7404811
CTTAAGT11000.00119664857.2861481
CCCACTT21501.9013896E-87.11670261
CTTCAGT25652.237357E-107.1015091
CCCTAAT10450.0058565176.972391
CTCCAAT36700.06.55157661
CCCAAAT32853.274181E-116.4322161
CCCAGCG27708.69295E-96.3129081
CTTCATT69300.06.30835341
CCTTAAC17551.05054656E-46.22747661
CTCGAAG21456.734437E-66.11424971
CTTTGAT71300.06.02921061
CTTCCGA40053.6379788E-126.00356771
CTCATGT17305.868946E-45.89630461
CTTAAAG30901.4235411E-85.8949421
Produced by FastQC (version 0.11.5)