FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578806
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAC450.00991658547.9847951
ACTAACC1150.00607007129.001472145
CCCAAGG3000.00493013214.3954391
CATGCAT4150.002971486412.1407321
TTCAGTG4200.003282010611.9806452
CTATAAT5150.001164920611.1809231
CCCATAT5300.001480576910.8644821
CTCCAAA6053.6989732E-410.7073521
CTTCGAT5750.002915538110.0142181
CTTTCTT13503.1358522E-67.99746561
CTTCACC8250.006243867.8520581
CCTTGAT9200.00189461017.8236081
CTTTAAA9700.00318392727.4203291
CTTTTAT9900.00388609367.27042341
CTTTTTA10250.00544923457.02216531
CTCTTTT11950.0032507456.62551551
CTTTTTT19000.00223642355.30358271
Produced by FastQC (version 0.11.5)