FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637606
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGA3305.036877E-415.3603181
CTTGCGT3350.00983428412.969481
CTATGAT4750.007777500410.6713781
CATCTTG5600.002283426210.31384855
CCTTGAT6400.00671308959.0516161
TCAAACT6550.00831924758.8179463
CTCTTTT11603.0869047E-58.1152421
CTTTAAA11001.4733274E-47.89959241
GTTGAAG9250.00194000837.80507478
CTTTTGT9950.0038497297.27768141
CTTCATT11050.00133627797.2085271
CTCCTTT10500.0064944526.8964691
CTTCTTT18102.5260391E-56.40114261