FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424589
Sequences flagged as poor quality0
Sequence length100-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1209.876105E-636.0733687
CACGTCT1550.001862681823.2649179
ACACGTC1850.00518661319.4991178
GCACACG2000.00813461118.0366846
AGAGCAC3000.004850920814.4293473
CCCTCTT3100.00550358914.1573641
CTTAGAA3650.001000778314.0280731
ATATAAG4900.00908025810.4494831
GGATAAG5750.0025483510.17688751
CTTTGAT9600.00245328437.61941531
CAATTAC4200.001359825.15333875-79
Produced by FastQC (version 0.11.5)