FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010226

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010226
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037622
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAAAG5702.1029466E-411.3821291
CCTACGG6400.00697269439.0108521
TCGACGG7350.0022646638.7873782
CTTCGAA8700.00991660457.45725631
CTCTGAA10550.00711262236.83287331
CAATAAT13300.00136879246.5040731
CTTTGAT17309.959294E-56.25030141
TCGATAT13900.00237576316.1933273
AATATTC17801.5766383E-46.04457865
GATAATA16955.472735E-45.92737532
CTTTCAT16000.00167949585.85705331
CTTCAGC17150.00383062135.46430641
AAGATGT15800.0098533575.44855983
CTTCCGG17200.00396452945.44842151
ACTTTTT18900.00219180765.3132594
CTTCCAT19300.00267761395.22909551
TTATTTC18000.007168795.1804284
CTTTATT18150.00745245955.16324231
AGATATT21450.00194138285.0160144
Produced by FastQC (version 0.11.5)