FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865748
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC6152.7284841E-1117.6066936
CCGATCT6802.1082087E-914.8612617
CTTCCGA9151.5845362E-610.258514
GCTCTTC9753.4073018E-69.6623871
GGTTGAA6600.008591448.7839891
TCCGTCC8150.0054432267.97392233
TTCCGAT12155.7299276E-57.7237595
TTTAATC9550.00265224987.5610782
TCTTCCG14255.296242E-57.0941483
ATTTAAT15651.7218321E-46.48275141
CTTTAAT14605.22728E-46.4526211
CTCTTCC13450.0015235786.4423762
Produced by FastQC (version 0.11.5)