FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706735
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT3201.9496481E-518.0999771
AACCCAA3305.22735E-415.2858445
CTTTAAC5802.3718539E-411.2344691
CAACAGG6455.0845774E-511.2247931
CTCAAAG7151.4462614E-410.1258611
ATTAAGA6800.0010274969.5823411
CTTTACT7050.00142910089.2425421
CTCCTCC7550.0026644938.6304531
CATCAAT13553.0452484E-68.01475051
CTTATTA9300.00199056537.7849371
CTTCAAG10100.00446323547.16830831
CTTCAAC10400.0059314366.96152971
TCAGAAA11950.00320865146.63424632
CAAGAAG12800.0062690316.22186761
CAAAAAA21753.5933685E-45.32597061
CTTCTTT25401.1011953E-45.13070251
Produced by FastQC (version 0.11.5)