FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1011910
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCA4600.006285910510.9826464
TCGATAC5750.002856563510.0402835
CTTCAAT14751.2551027E-109.8797281
GTTCGTT6809.699913E-49.6436331
CATGAAT8851.1506533E-49.0564181
CTTCGGA8709.683175E-48.3750571
TTGAATT17352.8328941E-87.90352632
CTTCAGT8350.00624166067.8534971
TACCTTC8550.0084094417.59626779
GTCAAAG12003.7516357E-47.28631
CCACCAT10150.0044051187.1786211
TTTCCCA11550.00221561926.8728137
CTTTGAT17009.176909E-66.85769371
CCTTGAT16203.457447E-56.7465741
CTCAAAG10850.0084084486.7154841
ATTCAGA14303.8029984E-46.62390951
TTGATGC13950.00231202096.20831633
CTCAAAA15900.0013705125.9573521
ATTGAAT14950.00450412985.84853551
CATCAAT21052.0629694E-45.538281
Produced by FastQC (version 0.11.5)