FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516422
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA3050.005120545614.3130351
CGAAACA4650.006407087710.9528071
ATAGCTG4650.006793446410.8669078
CAGATTT8155.1645064E-59.74307253
CTTCTGG6450.0068783519.024241
CTTTAAT9301.9978076E-48.6057771
Produced by FastQC (version 0.11.5)