FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences835936
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACAT2850.003160971715.391721
CCCATCT5005.4229044E-513.1599211
CTCCTCT4200.002893160612.1851121
CACTTAT5100.001046467811.3262823
CTCCGAT5400.001520139710.831211
GGACCCA1100.001948736810.514885105-109
CCCAAAT7251.5084288E-410.0842291
CCCAGTT7400.00203327028.8918391
CTTTGAT16609.08185E-87.9276631
CTTCAAC10154.8779155E-47.92332361
ACTTATT10000.00416071767.22050434
CTTCAAT14254.4956934E-57.18280271
CTATTAT11200.00139387087.18051241
CTCTTTG10600.00649236756.89723251
CTTTCAT20654.77532E-76.726891
CAACAAC14153.2060585E-46.71688131
CTCCTTT13250.00111734386.62134361
CTTCCTT14000.00208022336.2666291
CTTTTAT22351.8653773E-66.21522431
CTTTTCT20652.3159046E-56.01879641
Produced by FastQC (version 0.11.5)