FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659935
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2557.5977296E-519.6736567
ACACGTC2751.3561337E-418.2428468
CACGTCT3052.992019E-416.4484679
AGCACAC3406.833646E-414.7552425
AGAGCAC3508.5116137E-414.3336643
GAGCACA3750.001433301713.3780874
GCACACG4550.00610098311.0258966
CTTTGAG6608.2316255E-49.8168381
CAAACAT6600.0090646558.7260781
CTTTCAT11750.0027192116.7395031
CTTTTCA14050.00257276356.14862441
CTTTTTG15500.00116458666.0378831