FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779169
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2302.2553668E-623.226847
ACACGTC3453.8183134E-617.420138
CACGTCT3453.8183134E-617.420139
AGTTAGA2600.003135715115.4101147
AGCACAC4052.895102E-413.1947845
GCACACG4405.825012E-412.14526
AGAGCAC4950.001561298710.7964263
CCCACGA4350.007308580410.7616341
CTCACCT4350.00738920410.7457499
GAGCACA6006.8206445E-410.0204324
CCCAGCT6800.00211935988.8512181
GAAGAGC10350.00155277067.10757971
CGATATT10400.00165300377.06568964
CTTTATT12150.00114878746.60502431
AAGAGCA11200.00360321076.56141852
CTTTTAT12850.0021613466.24521731
TCACACG2808.432197E-46.2041625-29
AGAATAT15303.68125E-46.1126823
AAAAAGC15750.0034394295.51388933
Produced by FastQC (version 0.11.5)