FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences710680
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAGT3204.032406E-415.8167311
CTCAAAT6604.9190276E-612.0508431
CATCTGT4500.00526162911.2474531
CCCACAA4600.006197056311.0029441
CTTCTAT8003.500636E-610.8457591
CTCAAGA5700.002612820110.1480781
CTTCGAT8356.625563E-59.5252181
CTTTCGT6300.00597232449.1815951
TTGTGAT6500.0079320818.8696392
CAATTTC11754.1677995E-68.5841484
CTTCTCG7800.0036201948.3428911
CCTTGAT7850.00383511128.2897521
CTTTACG8000.0045485048.1343191
TTCAATT12459.036165E-68.1037872
CTTTATT16106.5258064E-88.0837951
CTTTGAT10004.6801343E-47.95355651
AGGTATT8450.0076768327.6734894
CTCAAAA9500.00248499687.6110581
CTTCAAT10507.889741E-47.57481581
CTTCCTT12004.092673E-47.2305061
Produced by FastQC (version 0.11.5)