FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586798
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCATT4602.5673731E-514.2473031
CTCCGAT5250.001241298611.09631251
CTCTTGC4700.00748106710.7271679
CACAGAT8304.9905702E-610.5281271
CTTCAAT9001.146771E-610.5183791
CTCAAGG4900.0093938910.4027921
CAATCCA5600.002326913210.2902014
AAACTTG6355.7449733E-410.2083055
CTAAAAT6100.0043167769.5501051
TGTCTGT8000.00470761778.1028426
CTTATTT9150.00160120237.95842031
CAAAGAT9300.00187933817.83005861
CTTTCCT9450.00219948967.70577241
CTTCATT10600.0067409536.86976861
CTTCCAT10800.0080761756.7425511
Produced by FastQC (version 0.11.5)