FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences602810
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATGG2053.4811007E-421.3810211
CTTATAC4953.0413194E-614.7579451
CCTCAAT4252.0396292E-413.7509321
CAACGAA6705.183696E-611.9935831
CCCTGAT4400.004119890311.6218811
CTTTGAA10103.0540832E-911.5725671
CTCATAT4450.004482520711.4912981
CTATAAT7251.2468396E-511.0837261
CTTCGGT6355.043133E-410.3538021
CTTGATT6700.0090921598.7226061
CCTTGAT7850.00349371678.3753681
CTTCAGC9602.6979792E-48.3705221
CTTCCGG13903.8491453E-67.8832911
AAAGGTT8300.0064141647.82863952
CTTATCA8350.00676889077.7817618
GGAAGAT11301.8170978E-47.7577161
CAACAAA10550.0062442076.9243441
AAGATTT12850.00671671426.18032653
CAAAGAA18250.0067879675.20369151
Produced by FastQC (version 0.11.5)