FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences577081
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCTA1850.006121488818.9492119
CTTATAA8551.0676487E-59.8770921
CTTTAAT7804.607553E-49.0223441
ATTTGCC7900.00533465067.99091436
CTTCAAG8000.00579825147.9171071
CAACAAA9955.9234665E-47.78007131
CTCCTTT9100.00212332357.73343751
CTTCCAT12005.574602E-47.03742841
CTTTTGT10550.0089573586.6705481