FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005010297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length128-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAACCATTCTCGGACTGCGTTCCCCGCTCTCTGGTGAAATAATCTTC12.857142857142857No Hit
CGATAATTTAATTTTGACATCAACTCTCTTAAAGCGGCTTTGTCAGCTTC12.857142857142857No Hit
AGTAGAATCAAGAACAAAACGGCAGACTGGGTGAACCTGCGCAACCTTTG12.857142857142857No Hit
CAAGCTGAAGGCGTTGCGCGACAAGATCGGCTACGTGCCGCAGCAGTCGT12.857142857142857No Hit
TCTGAAACTATATGGCTTTTACAAGTGGTAGTCCTTCTGCAGGACAACTC12.857142857142857No Hit
ATGGGATTTCAAGCCAGTCAGCGAGCAACAGATCCTCGCCTGGTTCACAT12.857142857142857No Hit
TCCAGCTCGTCGCAAGCGACATATCGCTTGCGACGAGCTTTTTCATTTTA12.857142857142857No Hit
ACATTACACTGTGGAATTACAAGAAAGACAATCGACCAGATTAAAAAGCA12.857142857142857No Hit
CTGAAGAACAAAAAATAAAGAACTATGACACTGGAAACAGAAACGGTTAT12.857142857142857No Hit
TCTCACTTTGTCCCGCCTTAGTAGTTATGATGATAGCACCTGCACCACCA12.857142857142857No Hit
GTTCCGTTGATGCTGGCATTTACAGCTCTTGCAGCATTAGGACAGGGACC12.857142857142857No Hit
ATGATTTCAGCAATACCGCCGGATTTCGTTAAACCAGCTGCGAACACTTC12.857142857142857No Hit
CCGTGGCGGTGAGGAAGGCGCTATTCGCCGGAATCTAGTGGAAAACCACC12.857142857142857No Hit
CAAAAGCATAGAAAAAAACAGCAAAAGAAAAAAGAATCAGGCAGGCCAGA12.857142857142857No Hit
TTTTAAGTTATTTATTCATTTCTACTTTGCTCTGACTTTAATCACCACAA12.857142857142857No Hit
GATCCAGTGGCTCGAGGATTACCTGAAAAACTACAACGGCGCGGTGCTGC12.857142857142857No Hit
TAAAGATTAGAAGAATTATTTCTAATCTTTTTTTGGAAATAATTTCCATG12.857142857142857No Hit
AAAACAGAAAGCACATTTTTTTGTGTATACACTTTTTCTTCTACATAATT12.857142857142857No Hit
CATCTTTTTGTCCTTTTGGAAGTCGCTCCAAAATATGCTCCAGCACACCA12.857142857142857No Hit
GATTCCCCTTACCGCGGTGCAGCCGTAAATAAACCGGAGGAGAACTTTAC12.857142857142857No Hit
AAGGAAACGGAGGTATTATGATGTTTACTTATGAAGAGATTAACCTGATG12.857142857142857No Hit
CCTGACAGAATAACATAGAAAGCTATAGCTTCCCAATAAATCTTCTTTTT12.857142857142857No Hit
CGGGCGAAGGAGGAGCCGGAGGCCCTCCACGCCGCCCCGGCGAGCACGCC12.857142857142857No Hit
CCTGGGCCTTGATGGAGGTGGCGCCGGACTGGTAGCTCTGCAAGTCATCG12.857142857142857No Hit
GATAAAATAATCGTTATTTTTATTTTTTGTATAATAACTACAAAACTTAT12.857142857142857No Hit
TTTCGACTTCGCGTAGGGATTTCCAAAATGCCGGTGTTATCGATGCCGGT12.857142857142857No Hit
GCTCAAGCGCGGCATACAGCAGTCCGGCCTGGACTTGGGCACGGCCAACA12.857142857142857No Hit
TCCATCATTCTGAACATCGGTTCAGAATGATGGATAGGAGAGCCCATATG12.857142857142857No Hit
GGGGCGCCATGCGCCGATCCTCTTCCCGTATACGGGCAAGCTGACCGACG12.857142857142857No Hit
CAATAATAGGATTGTTTTATCATATAGGTAATCGTAATTTAGTTCATCTT12.857142857142857No Hit
GGCCTCCCGCCCACGGCTCTGACCGGTGGTGGACGTTGGAAGCAGAGAAG12.857142857142857No Hit
AGCAAAATCAATAATAACCCCATTGAGATCAGAGTAGTTACATTTCTTAC12.857142857142857No Hit
GTTCAATTCTCCATGTTCTGCCACTTTCCTTGGATTCGGTTATCAACCCG12.857142857142857No Hit
TGAGAATAGATACATTCATAACTGTTTTCCGCTAAATTAACTTTCAGAAT12.857142857142857No Hit
ATTGTCTCTAAAGGAAAACTAACTACATTACTTCACAACTTAAAAACTGC12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)