FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8307280
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT25600.010.3119977
CACGTCT30300.010.0880979
ACACGTC36550.08.1729768
CTAGCAT10057.4298115E-47.61990451
CTTGGGT21901.1441443E-97.31150151
AGCACAC41800.07.1480155
CTTGGAT45050.06.9540951
AGAGCAC45300.06.74965763
CTAGAAT12459.6357893E-46.71018931
GAGCACA47900.06.5280484
CTTACCA41600.06.5267071
CTTGGGG31152.7284841E-116.48131041
CTTGGTT40550.06.18065551
CTTGGCT41850.06.15501551
CTTGAAT84850.06.15364361
CTTGCGA57100.05.97424271
GTTGGGA42100.05.95310161
CTTCCGA84500.05.7671891
CTTGGGA41105.456968E-125.7591711
CCTGGAT52150.05.7403321
Produced by FastQC (version 0.11.5)