FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850419
Sequences flagged as poor quality0
Sequence length100-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCAAT5256.928125E-613.6318071
CCAAAAC5852.8603818E-411.0103061
CTACAAT6457.040568E-49.9860921
GTCAAGA7550.0029601088.5311651
CGATGTT11101.9729238E-47.7037644
CTCTGCC9900.00411351547.22898861
ATCCGGG10100.0052108317.05542766
CCCGCAA10300.006045286.9482521
GTAAGAC3100.0086087946.601326145
Produced by FastQC (version 0.11.5)