FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3017509
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT8752.910383E-1113.9139477
CACGTCT10605.638867E-1112.161199
ACACGTC11653.4924597E-910.4503898
AGAGCAC14451.0913936E-1110.4085373
GAGCACA15052.564775E-109.51769354
AGCACAC16701.6556442E-88.1484575
GCACACG17604.1996827E-87.73177436
TTTAAAC25401.3126599E-45.0755632
Produced by FastQC (version 0.11.5)