FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518361
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGAT2100.009625195517.5182611
CTCAGAT2950.003809234614.964751
CTCAAAT4855.8545975E-412.1363621
CAATGAC4650.00589516911.0760611
CTTTAAT8204.865817E-48.9727681
CTTCAAT8506.961798E-48.6560821
CTTTATT10909.745811E-47.42517041
CTTTCTT14102.8336866E-46.78366661
CCCCCCC2450.0040529646.4894085105-109
TGATTTT17457.392295E-45.78797054
Produced by FastQC (version 0.11.5)