FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638673
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACT3050.00525431314.2582681
CCCAAAG4503.5366946E-412.8852491
CTTCAAG7451.8392442E-510.7016761
CTTATAT8801.1440409E-49.0599411
CCTCGAT6500.0075666368.9205581
CGCCTTC9302.0845715E-48.5728471
CCTTCAT9800.003289477.39587071
GAGAGCC3100.00741582745.591984730-34
Produced by FastQC (version 0.11.5)