FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568795
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAT5851.9106104E-512.3512791
TCCAATC5400.001719105810.67149452
CTTCAAT8951.1796601E-610.4951381
CTTGTTT8355.883083E-610.3839491
CCTCATT5750.002823784210.0528681
TTCAATT9452.6493863E-59.1469962
CATCGAC6400.0068326419.0318731
CTTCAAG6600.0087931978.758181
CTTTTCA13051.8286537E-68.305171
CCTCTTT9904.2287644E-48.0283321
TCAATTT9150.00178200557.8724133
CTTTTAT11302.0623919E-47.67309571
CTTTGAA9450.00237393387.646031
CTTCTTT18951.6190825E-87.62585541
CTTCCTT12054.3223266E-47.1955171
CTTTATT11600.00228399136.85176561
GTCTTTT10850.0091018946.6594461
TTTTTGT18452.4289572E-45.85476266
CTTTTTT24203.0335612E-45.0757631
Produced by FastQC (version 0.11.5)