FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998765
Sequences flagged as poor quality0
Sequence length100-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCC5802.2314365E-411.3095481
CTCCTAT7000.00126115459.3707691
CGACAGG6400.0063702959.1104691
CAACGAT10004.4994464E-58.746051
CTTAAAG9201.7482991E-48.7143621
CTTATGT6700.0092740738.7025381
CTCAAGA9402.2069077E-48.5289511
ACAAACT9352.3368852E-48.4839663
CAATTCA8700.0098831127.46003964
CCACCAT10900.00108041597.35524131
CTTCAAC15101.2869552E-57.24010751
ACGATTT10300.0056172087.00133133
CTTCCAT15201.09338835E-46.7129771
GTTTTAT20755.4869633E-76.67369371
TCAAAAC12200.00396536856.50205662
CTTTGAT14604.8787906E-46.489651
CCTGCAT12400.004205976.4654941
CTTAAAT12500.0045738916.413771
CTTCAAG18352.8164255E-56.35498671
TCAAATC12800.0065388186.19727233
Produced by FastQC (version 0.11.5)