FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694001
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA3050.00481138914.44893551
GGTAGAT4906.4809027E-411.9916321
TAACGGT4750.00804657910.6228029
CCAAAAC5950.00327397119.8754621
CTTAAAA6050.0037578879.7122321
CTCCGGT6050.0037578879.7122321
CTCAAAG6950.00109974719.511351
CCTCGAT7400.00194885568.9329561
CTCCGAA6600.0076918788.9028791
CTGCATT7550.00233851588.755481
CTTATAA7950.00373111678.3149531
CTTCAAA11100.00120603897.27870461
CTTCATA10300.0046956127.1309471