FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967536
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAAC3900.00179548612.9859081
CTCAGTT9352.227428E-59.2855691
CCCCCTT6550.0081887058.8366481
CCTCGAT17855.2514224E-98.1064481
CTCCTAT14801.2876626E-67.8216281
ATTTAGG11452.4925324E-47.5507366
ATTGGCG13101.4942253E-47.14966346
CTTAAAC10150.00472343767.12808471
CTCACAA10400.0059824976.95673661
CTTCAAG25251.375156E-96.87683731
CTTTAAT28606.184564E-116.83025031
CTTGAAA35250.06.77319671
CTCTATT15051.0593809E-46.7302381
CTCATAT21802.082852E-76.637621
CAACGAA18605.4236207E-66.612641
TCGCTAT12050.0035109916.57887553
TCCTATT16856.9624E-56.41560132
CTATGAG15801.9954324E-46.4107641
CTTTTAA37750.06.32464081
CTTATAG16102.545919E-46.29130941
Produced by FastQC (version 0.11.5)