FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879318
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2851.7499524E-417.6463687
ACACGTC3508.3575345E-414.3691848
GCACACG5800.00318284879.9097826
CCCAAAG7401.8441897E-49.8854541
CTCCAAT7652.576502E-49.56241
CCCAGAT7752.9354435E-49.4390141
CTCGAAG7400.002022968.8969091
GTTCTGT7500.0022852458.7782841
TTTCCCC8458.3332177E-48.5024777
CTTCAAG12105.0453073E-68.4639091
CTTTGGT7850.00345379818.3868951
CCCAGCT8900.00116569638.2193671
CTTCCAT13402.2443528E-68.1886971
CTTCTGA9850.00316141857.42663571
CTTTCTT19008.591378E-76.9302241
CTTTATT16052.871676E-56.83666941
CTTTTAT16504.2204345E-56.65021471
TTCCATT17852.3127675E-56.44070342
CAATATA15108.720424E-46.18541054
ATGATTT19003.7453414E-45.6720463
Produced by FastQC (version 0.11.5)