FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2425122
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTA6500.0084792938.7992591
AGTCCAC10400.0067527566.87045155
CTTGAAG28152.755769E-96.3493771
CTTCCAA21056.7416986E-66.11351
CTCTGAT16453.9188785E-46.08459471
CTCCAGT14400.00367104735.95783231
CTCGCAG15600.00137178935.95783231
CAACGAT21651.0625412E-55.9440731
CCCGCAG34209.458745E-105.8533091
CTTCCGA19609.743206E-55.83624361
CCCATAT18903.7381222E-45.67412571
CTTCGAT20752.209633E-45.51278921
CCCCAGC19656.318712E-45.45755621
CTTCGGA20750.00130891525.168241
AAGATTG28452.902602E-55.0230365
Produced by FastQC (version 0.11.5)