FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524546
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACAAC3800.001478174713.3226191
CTTCCGA4453.275794E-413.0018491
CTCATGG3950.001978255812.8166971
CTTATTT6850.0011084919.5022641
CTTGGAA7100.00153773089.1676771
ATACAGC6600.0089802478.7354636
CTTTCTT14204.9373404E-57.13041541
CTTTTTT15852.077877E-46.38813261