FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877265
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGAA7151.8078844E-49.9049971
CCAGCGG9653.9911972E-48.0728291
GAAATAG10157.327893E-47.62790735
CACAGAT9650.00355058777.33893541
CACAGGA9950.00478478067.11766051
ATGCCAA13001.8081315E-47.03847848
GGAAATA13904.0953307E-46.58354
TAGGTTG2600.00580830366.2694473110-114
CTTCCGG15450.00136524585.95902541
CTTTGAA17900.00129607475.53905151