FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1744254
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAAAT12559.777596E-68.0582561
CTCCAAC12054.350334E-47.1936771
AATGCAC12255.283997E-47.0725765
CTCTTAA10900.0095609286.6272031
CTTTAAT20756.4219967E-76.61442761
CCAACGA13600.00172186856.373811
AAGATTC19401.077066E-56.32673553
CTTTGAT25257.080598E-86.29387431
CCCATGA13900.00220096136.23624561
CTTAAAA22701.9263889E-55.72800541
CCCGCAA16550.00245431025.67416671
CCCCCCC3350.0028734815.59998740-44
AGATTCT18350.00139326145.5083874
CCTTGAT24255.5217366E-55.3618851
Produced by FastQC (version 0.11.5)