FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1203487
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC5107.4396667E-1018.408558
CACACGT5502.0336302E-917.0697487
CACGTCT5601.2287288E-512.89619
AGCACAC8203.853511E-711.4492215
CCCGCGA6103.7330127E-410.69871
GCACACG8054.5026176E-59.868326
AGAGCAC9350.00215349467.7238683
GAGCACA11352.1893332E-47.6354014
CCGCGAG8700.0096979977.4761252
CTCAAAA13501.9956226E-46.9827731
CCAGATT14400.0032509496.0224342
CGAAAAA19758.847803E-55.87450461
CTTCAAC15400.00660262025.65039541
CTTTGAT18650.0095535635.0545541
CTTCCAT18700.0098556395.04103951
Produced by FastQC (version 0.11.5)