FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922326
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATAG6002.9432346E-410.9765821
GTCAATT5450.001907005410.5406622
CTCAAAG10054.0271698E-710.193841
TTAATGG5650.002575190310.1664393
CCTCTAT5850.002935412310.0071391
CTCACAG5900.0031498189.9223351
CCCAAAT6000.00361977459.7569621
CTCAAGG6600.0079331878.8699651
ATAGTAT7300.0021181848.8521143
CTTAAAG6750.009535618.6728541
CAACTTC10801.3160176E-47.9778314
AACACTT8250.00638408637.83362872
CAAAGAG12951.0674565E-47.34597441
CTTCAAG10250.00464634277.13924031
CTTCGAT10400.005351067.03627061
CATTTAT12857.8090833E-46.8336691
CTTTGAT15408.8604755E-46.1772971
CTTTGAA14700.00355720875.97365051
CTTCTTT31151.5303158E-85.87297061
AATTCTT17256.8015774E-45.8272855
Produced by FastQC (version 0.11.5)