FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646737
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAT5709.027208E-714.015631
CTTAATG3200.007125887613.6174591
CCCATAA4955.2487645E-513.2048081
CTCCAAG4250.003321139411.9620031
GGTTCTT7450.00229449978.7736641
CTCCAAA8100.0048862048.0696051
CTTCAAA11352.0466984E-47.6785671
CTCAAAA8750.0097549527.4701491
CTTTCAA11450.00188715146.97721341
GTTTCAT10700.00757636876.78751851
CTTTCAG10750.0079254176.75594851
AAAGAAT19000.00230340435.29140572
CTTTTTC25108.68993E-55.20827151
Produced by FastQC (version 0.11.5)