FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339579
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAGAT5753.5288394E-916.3761061
AACGTAG4553.9972912E-412.7016435
CTCAGTT4907.293351E-411.825761
CCCACCT6656.916477E-510.8921471
CCCCTAT4900.00978290710.347541
CCCAATT7853.3059056E-510.14981651
AACATAG7954.3002455E-49.0868675
CCTTAAT9352.2294009E-48.5215031
CCCGCTT10559.0580725E-58.23881
CTCATAT11703.435182E-58.0480871
CTTTAAG9904.1293606E-48.0480871
CTTCGAT13101.6706237E-57.7409081
CTTCTAT15051.6270005E-67.7004951
CTTAATC8650.00902937.5363591
CTCCATT14709.634325E-67.39109951
CTTATAT16054.2671636E-67.22071271
CAACGAC10350.00564353776.9983361
CTCAAAT11400.00185721766.9891281
CTTTTAA18707.795297E-76.9721391
CTTTAAA19802.7136412E-76.95062071
Produced by FastQC (version 0.11.5)