FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005010573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005010573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1191327
Sequences flagged as poor quality0
Sequence length100-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAT13353.1779564E-89.128921
ATCGAAA14154.0785692E-46.5862561
CTCAAAA19159.819185E-66.36402461
CCCAAAA14100.0028113156.101181
CCTGCAT19056.191067E-56.02111151
CTTTGGA17458.044163E-45.7515421
AATTTAA19104.4423077E-45.60142375
CAACAAA21303.0800325E-45.3850791
CCAAAAT17650.00573592355.28021
CTTTAAA22506.687973E-45.0978751
Produced by FastQC (version 0.11.5)